Genotypic linkages of vp6 gene of human rotavirus isolates circulating In pediatric patients with acute gastroenteritis in haryana and comparison Of antigenic epitopes with vaccine strains

The major capsid protein, VP6 specify the sub-group specificity of Rotavirus and high titre of antibody are generated against VP6 gene. The Viral protein 6 (VP6), encoded by 6th segment of genomic double stranded RNA, is the main target for rotavirus detection both by serological methods and molecular techniques. A reverse transcription – PCR was standardized to amply the complete open reading frame (1194 bp) of human group A rotavirus VP6 gene. The genome segment 6 of local Rotavirus isolates from Haryana region were partially sequenced to analyze the variation within circulating rotaviruses and its epitopes were compared with that of vaccine strains.