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Genotypic linkages of vp6 gene of human rotavirus isolates circulating In pediatric patients with acute gastroenteritis in haryana and comparison Of antigenic epitopes with vaccine strains

Author:

Hari Mohan, Ray, P. and Gakhar, S. K.

Subject Area:

Life Sciences

Abstract:

The major capsid protein, VP6 specify the sub-group specificity of Rotavirus and high titre of antibody are generated against VP6 gene. The Viral protein 6 (VP6), encoded by 6th segment of genomic double stranded RNA, is the main target for rotavirus detection both by serological methods and molecular techniques. A reverse transcription – PCR was standardized to amply the complete open reading frame (1194 bp) of human group A rotavirus VP6 gene. The genome segment 6 of local Rotavirus isolates from Haryana region were partially sequenced to analyze the variation within circulating rotaviruses and its epitopes were compared with that of vaccine strains.

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