An experiment was conducted in the experimental field of Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh during the period from October 2012 to March 2013 to find out the effect of GA3 and pruning on the growth and yield of tomato. The experiment consisted of three doses of GA3 such as 80, 100 and 120 ppm with control; three different pruning levels such as 1, 2 and 3 stem pruning. The experiment was laid out in a RCBD with three replications. Both GA3 and pruning had significant influence on growth and yield contributing characters of tomato. At 75 DAT, the highest plant height (117.30 cm), maximum number of leaves/plant (75.30) and highest yield (29.03 t/ha) were recorded from GA3 spray at 120 ppm. At 75 DAT, the highest plant height (113.60 cm), maximum leaves per plant (67.00) and yield (28.11 t/ha) were recorded from 2 stem pruning. The combined effect of 120 ppm GA3 and 2 stem pruning performed the highest yield (31.89 t/ha) and lowest from G0P1. So, 120 ppm GA3 with 2 stem pruning may be used for higher yield of tomato.