Growth studies of bush mango- irvingia wombolu mildbr using in vitro techniques

Growth studies of Bush mango- Irvingia wombolu Mildbr was investigated using In vitro techniques with full, half and one quarter strength mineral components of Murashige and Skoog medium. This research was designed into 2 experiments. Experiment 1 was aimed at assessing the best medium strength and concentration of plant growth regulator for optimum growth of explants (axillary bud) of the species, using the concentration levels of 0, 1, 2, 3, 4, and 5mg/l of kinetin (Kin) and indole butyric acid (IBA) for shoot and root initiation, respectively. Experiment 2 was aimed at assessing the growth performance of explants of the species using the best medium strength (one quarter strength with 3mg/l kinetin) for further growth studies. In experiment 1, significant (P< 0.05) increase in shoot length of axillary bud explants was recorded in one quarter strength medium, while those of half and full strength media showed no significant increases. In experiment 2, leaf primordia of 1-2 layers as well as seedling and stump sprout explants were effective for optimum regeneration of the species. This study shows that optimum growth response of Irvingia wombolu explants can be achieved at lower medium strength (1/4 MS).