Bloodstains and bloodstain are some of the most common form of physical evidence encountered during the forensic investigation of incidents involving violence against a person. Blood and blood stains are a very important entity in medico legal practices as factors like source of blood and their stains help in solving the crime of violence, accidental cases. This study was carried out at Sam Higginbottom University of Agriculture, Technology and Sciences, and Ewing Christian College of Allahabad. In the present study, 5 different sample of blood were collected from central pathology lab and three different type of cloth were also been used. Cotton, Nylon and mixed cotton and nylon cloth were used. The purpose of this study was for the Identification of Blood stain on burnt cloth debris through FTIR and this was carried out with objective to find out the sensitivity of Luminol and Benzidine test for the identification of blood at higher temperature (500-10000c). Analysis on Cotton, Nylon, and Simple Cloth surfaces were considered. Blood is one of the important biological evidence commonly encountered by the investigators at the scene of violence crime. Thus it becomes essential to determine the sensitivity of reagents on blood stains on any surface. But in most cases of violence the presence of blood on the different Cloths surfaces leads to false positive or negative tests which in turns results in no option by the experts. So it’s very necessary to examine blood which assists the forensic analyst in an enhanced way.
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