Staphylococcus aureus (S. aureus) is a common gram-positive pathogen, which predominates as a global cause of bacterial infections. S. aureus uses the saeRS- two component system to control the expression of many virulence factors. SaeR the response regulator is one of the potent virulence factor that can cause many bacterial infections. So far the structure of saeR is not predicted. In this paper molecular modeling techniques were done using MODELLER 9v8 software to construct the 3 dimensional structure of SaeR protein with the help of a template which have the sequence identity more than30%. Structural characterization of this protein is important in rational drug design. The stereo chemical quality of the best model is validated by PROCHECK server and the Q site finder is used for locating, delineating and measuring concave surface regions on three-dimensional structure of the protein. Our results provide a framework for understanding the structure and the possible binding sites of saeR protein for drug targeting and the results were found to be reliable.