Invitro antioxidant, anti-inflammatory, inovo anti-angiogenic activities and virtual screening of phyto constituents of chromolena odorata

Chromolena odorata roots are used by traditional healers in Western Ghats for treating inflammation and tumor suppression. Hence the study tries to validate the use, by invitro antioxidant, anti-inflammatory and inovo anti-angiogenic activities. Virtual screening of active constituents of C.odorata was done using admet SAR an online web tool followed by molecular docking using autodock. Plant extract had a total phenol content of 30mg/g GAE. In comparison to the standard Ascorbic acid plant extract exhibited significant antioxidant activity by scavenging DPPH (IC50 104μg) and ABTS ions (IC50 84μg), reducing Ferric ions (IC50 50μg) and a Total Antioxidant Capacity of 27.5 mg/g Ascorbic Acid Equivalent. Percentage protection of HRBC by 300mg of plant extract was equivalent to that of 200mg of standard Diclofenec indicating anti-inflammatory activity with an IC50 value of 70μg. In inovo anti-angiogenic assay 500mg of extract disrupted the process of angiogenesis. Virtual screening highlighted the possibility of Chalcone, Kaempferide, Tamarixetin and Eupatilin becoming leads in future anti-inflamatory and anti agiogenic drugs.