
Polysaccharides produced by microorganisms are utilized for a variety of purposes, including the use in cosmetics and as food additives. More recently, polysaccharides have been exploited by the medical and pharmaceutical industries. The production and synthesis of these compounds is costly and time consuming. In the present work isolation of Aureobasidium pullulan was done from soil sample. The production of pullulan was done by using complex nitrogen medium with different concentration of soyabean oil. Pullulan yield was determined and optimum soyabean oil concentration in the growth medium for pullulan production was determined