Trichoderma viridae Pacilomyces liliaceae Verticilium lecani are the three Entamopathogenic fungi which are used to study the molasses as a source of their mass multiplication. Generally there are Fungi to control insects, weeds and to control other fungi. In nature every ecosystem exists in a balance. Growth and multiplication of each organism depends on the food chain, its predators, parasites, etc. In biological control system, these interrelations are exploited. The natural enemy of a pest, disease or weed is selected; its biology is studied for mass multiplication and utilizes the same to check the target pest. They are also specific in their action and perish once their feed (i.e. the pest) is exhausted. The result shows that mass production of fungus can be done widely by using molasses and shows that Trichoderma viridae shows Profused growth on molasses at 17th day. Pacilomyces liliaceae shows Profused growth on 22nd day after inoculation where as Verticilium lecani shows no growth after inoculation on molasses, but it shows Profused growth on PDA. The result indicates that molasses can used to grow fungal pathogens a s in India is a sugarcane belt where lot of sugarcane industries are located hence, molasses can be utilized to grow the same on large scale.