P53 gene Codon 72 polymorphism genotyping in Meningioma among Sudanese patients

Background: Meningiomas predominantly benign tumors that may result from an adverse effect of cranial irradiation and trauma. They account for 20% of all primary intracranial neoplasm. The incidence is likely to be much higher, since many benign meningiomas do not produce symptoms. The p53 tumor suppressor is a key regulator of cell cycle progression, such that its inactivation promotes increased cell growth and tumor genesis. Objectives: The study design to characterize the p53 codon polymorphism in Sudanese memimgioma patients & to correlate the p53 genotype with biological characteristic of the tumor. Material and methods: The study was conducted at Al-Shaab Teaching Hospital at The National Center for Neurologic Sciences (NCNS) during the period September 2009 and March 2011. It includes cranial meningioma Sudanese patients that were diagnosed based on radiology and histology. 70 meningiomas’ tissue samples were taken for DNA extraction. The amplification was carried out using PCR. Results: The genotyping analysis of 70 cases for p53 alleles revealed Arg/Arg was reported in 70% of the cases (30) whereas Pro/Pro seen in 17 (24.3%) and Arg/Pro was reported in only 4 (5.7%)