Background: This study was carried out to detect human cytomegalovirus (HCMV) in renal transplant patients in Khartoum state, Sudan, to show if urine gives a reliable result in HCMV PCR detection as plasma samples. Randomly, a total of 50 plasma and urine samples were collected from renal transplant patients at hospital of Sudanese renal transplant society during April to May 2012 Results: Urine and plasma together gave positive CMV result in 4 patients, while 2 patients were detected by plasma only, and one patient was detected by urine sample only. Analysis of result give a significant relationship between the two samples (p =0.00). Conclusions: The results of present study indicated that urine can be used for PCR detection of CMV as plasma sample, but further study need to be done for more validation.