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Pcr-rflp analysis of mitochondrial 12s r rna gene for identification of meat and meat products in some avian species

Author:

Ismail A. Abdul-Hassan and Ghufran J. Al-Aqeeli

Subject Area:

Life Sciences

Abstract:

A molecular approach using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to distinguish four avian meat species in addition to pig. The partial 12S rRNA gene was amplified with universal primers. The amplified fragments (456 bp for all species studied) were digested with Acil restriction enzyme.The results showed that each of chicken, turkey, quail, duck and pig species exhibited a unique specific RFLP pattern that allowed its identification and discrimination from others. Meat products studied were confirmed through PCR-RFLP of 12S r RNA gene and showed conformity with the declared label.PCR-RFLP analysis of mitochondrial 12S r RNA gene was found to be an accurate and authentic method for meat species identification.

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