The study was aimed to evaluate the anticancer activity of the seed coat of Borassus flabellifer on the HeLa cell line. The seed coat of Borassus flabellifer PBS extracts were tested for inhibitory effect on HeLa Cell Line. The cytotoxicity of Borassus flabellifer on HeLa cell was evaluated by the MTT assay. The PBS extract of Borassus flabellifer has shown significant cytotoxicity on HeLa Cell Line in concentration range between 32 μg/ml to 750 μg/ml by MTT assay. Our preliminary studies on HeLa cell line indicated that even the lower concentrations of plant extract tested showed significant antiproliferative activity.