Three hundred female patients attending infertility clinic of obstetrics and gynaecology, RMMCH were enrolled in this study for a period of two years. Exclusion criteria included vaginal bleeding and pregnancy. Two high vaginal swaps were taken from each group of patients in three groups, Group1 comprising of 100 primary infertility patients, Group 2 comprising of 100 secondary infertility cases and Group 3 of 100 idiopathic infertility patients. Specimens were processed anaerobically and the results were analyzed. Bacteroides, peptococci, peptostreptococci, fusobacterium, prevotella, veilonella and mobiluncus were the anaerobic organisms found in bacterial vaginosis. A significant raise of anaerobic organisms (70%) were found in idiopathic infertility cases against lesser positive anaerobic organisms (56%) in secondary infertility. Bacteroides were found predominantly in 30% of idiopathic infertility cases as against 22% of positive cases in secondary infertility.