Background: This study was carried out to determine the prevalence of WNV IgG and IgM among multi blood transfused patients in Khartoum, State Sudan. Material and Methods: Ninety one sera samples were collected from multi blood transfused patients from Khartoum teaching Hospital during the period from August to November 2013 and subjected to Enzyme Linked Immunosorbent Assay (ELISA) to detect WNV IgG and IgM antibodies. Results: Out of ninety one 50(54%) were positive for WNV IgG antibodies, while 11(12.1)% were positive for WNV IgM antibodies. Conclusions: In conclusion, the incidence of WNV among multi blood transfused patients in Khartoum State, Sudan was documented through detection of WNV-specific IgG and IgM antibodies. Further study using various diagnostic methods should be considered to determine the prevalence of WNV disease at the national level.
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