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Production, Characterization, Kinetic Studies Of Glucoamylase Through Solid-State Fermentation By Aspergillus Niger Using Agricultural Residues As Substrate

Author: 
Sarojini R., Deepika K. and Rangabhashiyam, S.
Subject Area: 
Life Sciences
Abstract: 

In the current study the Glucoamylase production was carried out by Aspergillus niger on the solid state fermentation of Agricultural residues. The Agricultural residues, subjected as the substrate for glucoamylase production are Ricebran, Cotton seed flour, Coconut oil cake, Wheat bran, Groundnut oil cake, each in a separate conical flask. Aspergillus niger was incubated in those different agro residues for 5 days at 37ºC for finding the suitable substrate favouring the better enzyme expression with optimum characteristics features. The cultural conditions were optimized for the enzyme production, 20% [50/250ml-flask] was found to be optimum volume of the medium. With comparison of all the five agro residues “Ricebran” showed high yield of (7µg/ml) Glucoamylases in SSF. Optimum enzyme activity was observed at 45 ºC at pH 5.2. The kinetic studies of Michaelis Menton parameters includes the estimation ofVmax and Km, were 20(µg/ml.min), 10(g/l).

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