Rhizosphere fungi of 12 basmati rice varieties collected from 11 different localities of North India were isolated, Total 207 fungal strains were obtained that were identified and their pure cultures are maintained. These strains were screened for 2AP synthesis; the principle basmati aroma compound on the synthetic medium proposed by Rungsardthong. 12 fungal species namely Aspergillus terreus, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Nigrospora sphaerica, Nigrospora oryzae, Trichoderma sps. Penicillium sps, Rhizopus oryzae, Fusarium oxyporum, Monilia sitophila, Thielavia terricola were found to synthesize 2AP. 2AP synthesizing fungus (Aspergillus terreus) was taken for qualitative analysis by GC-FID.