Poisons like pesticides and insecticides are commonly encountered in suicidal/homicidal cases in India and abroad. Out of the large number of pesticides used for criminal activities Organophosphorous (OP) group of insecticides are largely reported in forensic toxicology cases. Qualitative and the quantitative analysis of the forensic toxicology samples are very important for the medico-legal cases to provide justice to the victims. In the present study we analyzed some of the quinolfos, chlorpyrifos & malathion insecticides in spiked biological tissues. For the qualitative analysis screening by colour/spot test & TLC was performed by using different solvent systems. Further quantitative analysis was done by using HPTLC-densitometer for the confirmatory results.