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Rflp-pcr analyses to capture polymrphism of alad gene on individuals exposed to lead compound, mda level and haemotological profiles

Author: 
Hernayanti, Hadjid R Mardihusodo and Nurtjahjo D Sasongko
Subject Area: 
Life Sciences
Abstract: 

Lead (Pb), is a toxic compound thus able to generate free radical and inhibit the δ-amino levulinic acid dehydratase activity, thereby affecting heme production. Due to its potential impact on human health, this study aimed to examine the polymorphism of the δ-ALAD gene in individual exposed to lead and to investigate its relationship with malondialdehydeand haematological profiles. Using a cross-sectional design, Venous blood were collected, genomic DNA was extracted and amplified using an RFLP PCR with the Msp-I restriction enzyme to for detect δ-ALADgenotype. Bloodleadconcentrations and haematological parameters were measured using atomic absorption spectroscopy (AAS) and a Haematology Analyzer, respectively, while MDA level were determined by TBARS method. One third of the subjects exhibited a polymorphic pattern for the δ-ALAD genewith band at 916 bp, 582 bp and 334 bp, whereas the remaining show inversely, a single at 916 bp. Individuals with the polymorphic genotype showed significantly higher blood-lead level, hemoglobin, erythrocyte and leucocyte counts compared to non-polymorphic individualsp<0.01). These findings suggest that the δ-ALAD gene polymorphism may impact δ-ALAD activity, increase oxidative stress as indicate by level, but decrease hemeproduction leading to anemia.

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