Knowledge of the diversity and ecology of fungi in forest stands is important for understanding the interactions between these microorganisms and plant communities. The objectives of this study were to collect, identify and classify macroscopic fungi (Agaricomycetes) found in forest fragments and adjacent areas in the Forest Research Center of the Fundação Estadual de Pesquisa Agropecuária (FEPAGRO) and surroundings, in the state of Rio Grande do Sul, Brazil. This search was conducted from April to June 2013, in fragments of pine, black wattle, eucalyptus, native species, mixed stands, as well as in grasslands near forest fragments. We found 40 species of fungi, 29 considered ectomycorrhizal, eights aprophytic and three lignocellulolytic species. Among the ectomycorrhizal fungi were species belonging to nine different genera: Amanita, Descomyces, Lactarius, Pisolithus, Ramaria, Rhizopogon, Russula, Scleroderma and Suillus. Seven saprophytic fungi genus were collected: Calvatia, Gymnopilus, Lepiota, Lepista, Leucoagaricus, Macrolepiota and Stropharia. Among lignocellulolytic fungi were three species of the Ganoderma genus. Samples of specimens of fungi collected in this study were stored to allow molecular studies. This scientific survey confirmed the existence of a large variety of macroscopic fungi in the forest fragments of the Forest Research Center (Rio Grande do Sul State, Brazil), demonstrating the potential of these sites as a source of mycorrhizal, saprophytic and lignocellulolytic fungi besides the importance of these habitats for the preservation of genetic heritage.