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roduction, optimization and molecular characterization of glucose isomerase by enterobacter agglomerans isolated from soil

Author:

Nobel Surya Pandidurai, R., Kalaiselvan, P.T., Mukesh Kumar, D.J. and Gnanaraj, M.

Subject Area:

Life Sciences

Abstract:

A bacterial strain that produces glucose isomerase was isolated from garden soil and identified as Enterobacter agglomerans. Enzyme activity was screened by the formation of cherry red colour and the chemical assay was determined by glucose oxidase enzyme using fructose as substrate followed by the measurement of the amount of glucose. The highest level of extracellular glucose isomerase obtain (41U/ml), temperature around 37°C, pH.6, xylose as a carbon source, peptone as a nitrogen source and incubation time for 36 hours for its higher enzyme productivity. The SDS-PAGE analyses of crude enzyme shows multiple bands, more over along with glucose isomerase some other protein can be produced by the organism.

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