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Role of polymorpho nuclear leukocytes (pmns) in malarial infection and their correlation with apoptosis

Author:

Singh, V. K., Srivastava, S. and Singh, K. P.

Subject Area:

Life Sciences

Abstract:

Neutrophil from the malarial (Plasmodium yoelii) infection was isolated by Ficoll-Hypaque and Dextran sedimentation method, the viability of neutrophil was assessed by trypan blue to be 95-98%. The viable neutrophil follows apoptosis and modulates the myeloperoxidase (MPO) activity. Our data suggesting that, the malarial infection inhibits MPO activity of neutrophil. During P. yoelii infection, the induction of apoptosis was found in neutrophils using FACS. This data suggesting that this apoptosis may be initiated by leukocyte hemozoin coming after phagocytic ingestion of parasite-libratedhemozon (HZ) in peripheral blood.

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