CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

RP-HPLC & hptlc analyses and standardization of payapro™ premix-a polyherbal veterinary galactogogue

Author: 
Anirudh Sharma, Uma Ranjan Lal, Pushap Lata and Deepak Thakur
Subject Area: 
Health Sciences
Abstract: 

Objective: The aim of the present study was to standardize Payapro™ Premix by quantitative estimation of apigenin and glycyrrhizin by reverse phase high performance liquid chromatography (RP-HPLC) and high-performance thin layer chromatography (HPTLC) methods. Payapro™ Premix is a polyherbal formulation used as galactogogue containing seven herbs including Cuminum cyminum and Glycyrrhiza glabra. Materials and methods: RP-HPLC and HPTLC methods were developed for the standardization of Payapro™ Premix by quantitative estimation of apigenin and glycyrrhizin, the bio-active constituents of Cuminum cyminum and Glycyrrhiza glabra respectively. The developed methods were validated on various parameters, including linearity, precision, accuracy, LOD, and LOQ as per ICH guidelines. Results: The RP-HPLC and HPTLC analysis methods were selective for the polyherbal formulation. Both the methods had specific linearity range with regression coefficient ≥ 0.995. The apigenin and glycyrrhizin estimation methods were precise (% RSD 0.7 and 0.4), accurate (average recovery values were 96.28 % and 95.83 %), LOD (sensitive) (0.0054 µg/mL & 0.016 µg spot-1), LOQ (reliable) (0.0162 µg/mL & 0.048 µg spot-1), respectively. Methanolic extracts of polyherbal formulation showed the presence of significant amount of apigenin (50 µg/g) and glycyrrhizin (1200 µg/g) using developed methods. The comparison of different batches for these markers was found to be uniform which ensures their quality. Conclusion: The present work emphasized on standardization of the polyherbal formulation by determination of bio-active marker constituents. The developed methods can be used to standardize other samples containing Cuminum cyminum and Glycyrrhiza glabra.

PDF file: 

CALL FOR PAPERS

 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

CHUDE NKIRU PATRICIA
Nigeria
Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran