
Protein profiling during ex vitro multiple shoot regeneration has been reported in three Rhizophoraceae mangroves viz. Bruguiera parviflora, Kandelia candel and Rhizophora apiculata occurring in Odisha. The effect of exogenous application of NaCl and proline on protein changes was evaluated during the process of multiple shooting in treated and non-treated hypocotyls. The polypeptides of the molecular weight (MW) 29 & 20 KDa appeared to be common in untreated hypocotyls of all the studied species. R. apiculata synthesized an unique polypeptide of MW 14 KDa in normal hypocotyls. In K. candel, NaCl induced synthesis of 26 & 17 KDa polypeptides. Proline facilitated synthesis of new polypeptides of MW 43 & 32 KDa in R. apiculata and 40 & 33 KDa polypeptides in B. parviflora. Thus, altered protein profiles be treated as possible markers for evaluating species-specific ability towards ex vitro multiple shoot regeneration in Rhizophoraceae mangroves as influenced by salinity and proline.