Bioinsecticides have several advantages over chemical insecticides in the control of major vector borne diseases. The secondary metabolites from plants are generally used as bioinsecticides. The aim of the present work is to evolve a biological or ecofriendly control over the mosquitoes using flower extracts from Spilanthes calva by evaluating the effect of phytochemicals in the survival of mosquito larvae. Both aqueous and solvent extracts were tested (LC50) against the mosquito larvae. The concentration of the extract used was in the range of 1-120ppm.Column chromatography was used for the isolation of crude extract. Each fraction was analyzed using TLC. From the results it was seen that petroleum ether fraction is more effective in controlling the mosquito larvae (Culex quinquefasciatus).