The troponins are regulatory proteins found in skeletal and cardiac muscle. Most of the early markers identified were enzymes, and as a result, the term "cardiac enzymes" is sometimes used. However, not all of the markers currently used are enzymes. Cardiac biomarkers should be measured in all patients who present with chest discomfort consistent with acute coronary syndrome. Elevations of cardiac enzyme levels should be interpreted in the context of clinical and ECG findings. In this study, cTnT levels of 50 AMI patients were compared with the 25 normal blood donors. We observed 20 times increase in cTnT levels in AMI patients as compared to normal donors. Cardiac troponins T and I are the preferred markers for myocardial injury as they have the highest sensitivities and specificities for the diagnosis of acute myocardial infarction. Peak circulating enzyme levels tend to occur earlier and are often higher following successful thrombolytic therapy.