Stevia rebaudiana is a South American plant native to Paraguay that traditionally has-been used to sweeten beverages and make tea. Previous phytochemical studies have revealed this genus to be rich in secondary metabolites including glycoside, flavonoids, lactones and terpenoids. A phytochemical study was conducted on the leaves of S. rebaudiana.Lupeol-3(4’-hydroxy) dodecanoate were isolated and identified. The separations of the chemical constituents (triterpenoids) were carried out using different chromatographic techniques including column chromatography and structures of compounds were confirmed by spectroscopic techniques including nuclear magnetic resonance as well as mass spectrometry. Separation, isolation and characterization of novel secondary metabolites (triterpenoid), which may act as prototype for the preparation of new drugs with low side effects and better efficacy.
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