
Introduction: A child to use toilet is one of the pivotal milestones of development, it is necessary to adapt to the social and cultural value of the society. Late toilet training may lead to dysfunctional elimination. Toilet training should start only after the child must be both physically and emotionally ready for toilet training. According to psychosexual theory of Sigmund Freud the correct time to start toilet training is toddler period especially 18-24 months. Aim: To assess the knowledge and practice of mothers regarding toilet training and to prepare guidelines to enhance the knowledge and to improve their practice for toilet training among toddlers. Material and Method: Descriptive study with non-experimental research design. The sample of 100 mothers was selected by using simple random sampling technique. Self structured questionnaire and check- list were used to collect the data from mothers of toddlers. Results: The data was analyzed by using descriptive and inferential statistics in terms of mean, SD, ‘t’ ANOVA and Percentage. The finding of study revealed that majority of mothers i.e 64% had average level of knowledge but showed adequate level of practice i.e 73% regarding toilet training among mothers. Conclusion: hence it was concluded that majority of mothers had average knowledge and their practice level is adequate regarding toilet training in toddler.