Botox Cosmetic (Botox) is a formulation of the neuromuscular blocking agent botulinum toxin type A (BTX-A). The use of botox for enhancement of facial esthetics is most common procedure currently undertaken in clinical practice all around the globe. Dentists are highly knowledgeable and precise regarding facial anatomy and therefore it seems reasonable for them to be at the forefront in providing these services. This review encompasses the pathogenesis of facial lines and wrinkles, toxin overview, preparation, uses, complications and contraindications of botox therapy and provides the practitioners in-depth and current knowledge of use and handling botox for facial rejuvenation. Factors unique to each area are presented, highlighting the key elements that can increase the likelihood of a successful outcome.