Evaluation of antibacterial activity and phytochemical analysis of crinum asiaticum

Aqueous and ethanol extracts from the leaves of crinum asiaticum plants were investigated for their antibacterial activity against gram negative bacteria Pseudomonas aeruginosa (ATCC10031) Klebsilla pneumoniae (ATCC 10031) and Escherichia coli (ATCC 25922) and gram positive bacteria Staphylococcus aureus (ATCC 11632) and Bacillus subtilis (ATCC 23859) using the agar well diffusion, disc diffusion and broth dilution methods. These extract ranged between 0.5 -1.5 mg/ml. The patterns of inhibition varied with the plant extract, the solvent used for extraction, and the organism tested. The different concentration of ethanol extract was significantly differed when compare to their aqueous extract. The maximum zone of inhibition and minimum inhibitory concentration (MIC) was found to be 1.5mg/ml. The minimum inhibitory concentrations (MIC) value of the ethanol extract of Crinum asiaticum were in the range from 0.187 mg/ml to 0.375 mg/ml. Phytochemical analysis, more than 22 compounds have been identified by Gas chromatography with mass spectrophotometry, n-Hexadecanoic acid (22.44%), 9, 12, 15-octadecatrienoic acid (15.42%), 9, 12-octadecadienoic acid (14.78%), 9, 10–Anthracenedione 2-amino (7.65%) and phytol (7.43%) are the major present components. We conclude that the leaf of Crinum asiaticum is a natural source of new antibacterial compounds.