Assessment of tumor associated tissue eosinophil (tate) in oral squamous cell carcinoma using carbol chromotrope stain

Eosinophils are known moderators of innate and adaptive immunity. However, studies focusing on the role of Tumor-Associated Tissue Eosinophils (TATE) in oral squamous cell carcinoma are limited, keeping which in mind, the present study was conducted. AIM: To evaluate tumor-associated tissue eosinophilia in mild and moderate oral squamous cell carcinoma. METHOD: 30 formalin-fixed paraffin-embedded blocks of histopathologically proven oral squamous cell carcinoma cases were retrieved from the department archive. Two sections of 4μm of each block were subjected to staining by routine Hematoxylin and Eosin stain and Carbol Chromotrope stain. TATE was graded based on the modified classical counting method (Alkhabuli and High, 2006). Statistical analysis was done using SPSS version 21, and P<0.05 was considered statistically significant. RESULT: Statistically significant association was found between TATE count and oral squamous cell carcinoma (P<0.0001). CONCLUSION: The positive correlation between TATE count and increasing oral squamous cell carcinoma grades could indicate its role as a prognostic marker. We further concluded that Carbol chromotrope couldbe a better stain for TATE than routine hematoxylin and eosin.