Background of the study: Blood sample collection is an invasive procedure and is carried out for diagnostic purposes; standardized technique in withdrawing blood sample should be followed to reduce pain and psychological trauma. Therefore it is essential to follow the Standard Operative Procedure for collection of blood specimen to get accurate laboratory results. Methods: The design adopted for this study was descriptive design. The study was conducted in pediatric unit. Data collection period was for one month. Samples were Health care workers in Pediatric Unit of selected hospital. Purposive sampling technique was used to select the participants for the study. Sample size was 45. The tool used for data collection was observational checklist. Results: The findings show that compliance of Doctors in following standard operating procedure in blood sample collection was 79%.The compliance of staff Nurses in following standard operating procedure in blood sample collection was 89.8%.The compliance of staff Nurses who assisted phlebotomy was 75% and student Nurses who assisted was 88.9%.The compliance of Attendants in following standard operating procedure in transportation of samples was 96.6%. No significant association was found between the compliance and demographic variables of Health care workers. Conclusion: The study findings revealed the health care workers do not have 100% compliance in following standard operating procedure in blood sample collection. Health care workers need to be reinforced about their accountability in minimizing errors related to blood sample collection and transportation.
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