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Crispr delivery systems and its applications

Author: 
Sowmya, K.L. and Dr. Ramalingappa, B.
Subject Area: 
Life Sciences
Abstract: 

Discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR based prokaryotic adaptive immune system and its repurposing into a potent gene editing tool has revolutionized the field of molecular biology and generated excitement for new and improved gene therapies. Additionally, the simplicity and flexibility of the CRISPR /Cas 9 site specific nuclease system has led to its widespread use in many biological research areas including development of model cell lines, discovering disease mechanisms, development of transgene plants and animals. In this review we present a CRISPR delivery systems including physical delivery methods like microinjection, hydrodynamic delivery and viral vector delivery methods like Adeno Associated Virus (AAV), Lentivirus (LV) and Adenovirus (AdV) and its applications in different fields.

PDF file: 

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