An efficient plant tissue culture methodology plays a significant role in genetic improvement associated with important regulatory genes. The present investigation was undertaken to develop a tissue culture system for cumin variety GC-2. Hypocotyl as an explant was inoculated into media supplemented with various concentrations of plant growth regulators for callus induction, proliferation, shooting and rooting. The MS (Murashige and Skoog) media with 0.1 mgL-1 BA (Benzylaminopurine) gave rise to callus induction, whereas upon increasing the concentration of BA (1 mgL-1) callus proliferation was observed. The growth regulator free B5 basal media supported shoot and root induction simultaneously.
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