The purpose of the study was to examine the effect of plyometric training on various surface on selected physical fitness variable among college women soccer players. To achieve the purpose 36 inter- collegiate women soccer players were selected as subjects. Their age ranged between 18 and 24. The selected subjects were randomly segregated into four groups of nine each. Group-I underwent plyometric exercises on the sand surface, group-II underwent plyometric exercises on the soil surface, group-III underwent plyometric exercises on the grass surface and group-IV acted as control. The investigator has selected speed, agility and endurance as physical fitness variables. The experimental variable used in the study was plyometric training on various surface (Sand, Soil and Grass). A 12-week plyometric training programme was developed using three training sessions per week. The results show that, 12 weeks of sand, soil and grass surface plyometric training improved the speed, agility and endurance performance significantly. Considering the endurance, performance, sand surface plyometric training is better than soil and grass surface plyometric training. However, when comparing soil and grass surface plyometric training groups, soil surface is better than grass surface.