Studies on larvicidal activity of gloriosa superba on the developmental stages of the chickungunya mosquito, aedes aegypti

In the present study the larvicidal effect of botanical insecticide, Gloriosa superba against Aedes aegypti was administrated. The plant extract showed higher mortality in lower doses and it exhibit better protection against the different developmental stages of Aedes aegypti. Based on the probit analysis,the LC50 values and 95% upper and lower fiducial limit and regression values of the plant extract on Aedes aegypti were also noticed. The treatment of seed extract against III and IV instar larvae of Aedes aegypti showed that III instar larvae are more susceptible than IV instar larvae. These morphogenetic abnormalities are commonly caused by botanical extract and the disturbance from the growth regulating hormones. It is because the active principle compounds such as terpenoids, alkaloids, flavonoids, phenols and tannins present in this plant. Activity of alkaline phosphatase has been drtermined during different hours of larval development of Aedes aegypti. The activity has been determined during III instar mould to IV instar mould with a period of 24 hours in Aedes aegypti, activity increased from 35ppm to 40ppm. It is therefore suggested that Gloriosa superba derivatives can be effectively used as a biocide for vector control operations.