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Tissue typing methods to assess the compatibility and clinical outcome in the light of immune monitoring criteria following organ transplants

Author: 
Siddhardha Solosan, S., Euphrasia Latha, J., Lakshmi Priya, N., Gopalakrishnan, N., Rosy Vennila, P., Selvi, R. and Secunda, A.
Subject Area: 
Life Sciences
Abstract: 

In the present investigation an assessment was made on the potential renal transplant subjects involving HLA typing of the donor and recipient (Table 5.1 - 5.10) , screening for alloantibody against HLA, and obtaining a history of sensitizing events. HLA typing ideally includes A; B; C; DRB1; DRB3,4,5; DQB1; DQA; DPB1; and DPA; but this is not always performed. The single antigen bead (SAB) solid-phase assay is most commonly used as the first line of screening for alloantibody, but multi-antigen screening beads can also be used. In the tables mentioned above, 5.1 - 5.5 imply live donors, while the tables 5.6 - 5.10 are based on cadaveric donors. Furthermore, the criteria such as CDC HLA crossmatch, PRA Class - I and II HLA, PCR-SSOP are pre transplant data, while the remaining such as DSA IgG HLA Class-I and II, SAB-FCXM HLA Class - I and II , NGS - Illumina MiniSeq are post transplant data. Besides in the current pursuit, more importance was given to renal transplant data, than liver, heart and lung. The data were also analysed in the light of advanced statistical and computational tools and expressed as graphics and tables (5.21 - 5.24).

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