In the Present research work we were collected infected samples from the field and directly brought in the laboratory of Botany, Shikshan Maharshi Dnyandeo Mohekar Collage Kalamb dist. Osmanabad. Near about 10 fungal species were collected in kharip, 2014 from the soybean crop from all over the Marathwada region such as Fusarium, Alternaria, Penicillum, Rhizoctonia, Tricoderma, Verticillum, Aspergillus, Helmenthosporium, Collatotricum etc. Isolation of these fungal species was done by PDA (Potato Dextrose Agar) petridish method under aseptic condition (Laminar Air Flow) and maintains pure culture in incubator which was set at 240C. Soybean plants cultivated in the plastic bags and growing under controlled and disease free condition in the green house. After 40 days, well growing plant was taken for spraying the fungal suspension. Disease free plant was selected and makes wound on the plants part, with the help of needle and blade. Premade suspension of fungus spread on disease free plant and kept for further results with the control sample. Lastly result were noted + 25%, ++ 50%, +++ 75% and ++++ 100% as like in table 1.
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