Production of bioethanol after hydrolysis of lignocellulosic biomass into sugars using zymomonas mobilis and saccharomyces cerevisiaeisolated from wine sample

Cellulase is an inducible enzyme complex, produced by a number of bacteria, actinomycetes and fungi including Trichoderma species and Aspergillus species etc. Corn husk which is composed mainly of cellulose. It is a major component of agricultural and domestic waste. It can be converted to bioethanol, alternative source of energy. Production of cellulase was induced in the fungi Aspergillus nigerby growing it in mineral salt medium and alkali pre-treated corn husk. The product of hydrolysis was fermented with the help of Saccharomyces cerevisiae and Zymomonasmobilis. The cellulosic hydrolysate yielded 1.78 gl-1 sugars, from which got 9.10 gl-1 ethanol, which obtained after fermentation using Zymomonasmobilis. This is compared to ethanol yield produce by Saccharomyces cerevisiae which was just 8.20 gl-1. Thus, our study has shown that corn husk hydrolyzed with cellulase extracted from A. niger with the help of Z. mobilis and S. cerevisiae isolated from red wine could be a perfect source of bioethanol.