Cell death induced by hydroethanolic extract of artocarpus hetrophyllus seed in hela – cervical cancer cell line

Artocarpus hetrophyllus has been traditionally used for treatment of diverse diseases and it possesses antibacterial, anti-inflammatory, antidiabetic, antioxidant, and antipyretic properties. Artocarpus hetrophyllus has a potent cytotoxic effect on human cancer cells. In this study, we reveal the cytotoxic effect of hydroethanolic extract on HeLa cell line. The dose-dependent effect of extract was measured by MTT assay and nuclear changes during apoptosis was assessed by Ethidium bromide Staining. Phytochemical screening and individual components present in the extract were also identified by comparison of both mass spectrum and their GC retension data. The IC50 values of hydroethanolic extract was found to be 60.25 µg/ml. Most of the cells were observed for nuclear changes by Ethidium bromide staining.Preliminary phytochemical screening revealed the presence of possible role of secondary metabolite. The quantitative data of 25 volatile constituents was obtained by peak area and the retention time were calculated for all the compounds. The major volatile components identified in the extract of Artocarpus hetrophyllus were found to be 1-Dodecene(17.7 %) 1-Tetradecene(17.2%) and Hexadecene (11.2%) respectively. The overall result indicates the promising baseline information for the potential uses of the hydroethanolic extract of Artocarpus heterophyllus seed spermoderm as an anticancer agent.