The aim of this study was to evaluate the physiological performance of wheat seeds derived from plants subjected to desiccation with different herbicides and seed moistures, before and after storage. A randomized block experimental design was used in a 4 x 3 factorial, with three herbicides and application absence and three seed moisture contents at application. 1000-seed weight, germination and first count, germination speed index, emergence and emergence speed index, and accelerated aging and electrical conductivity were assessed. Seed vigor is reduced by herbicide action when applied in 40% moisture, what is related to the active principle. Germination and vigor of wheat seeds from plants subjected to herbicide application in different seed moistures have similarity when assessed before sowing and after storage.