
The present study was carried out to determine the effects of sub-lethal concentration (96 h-LC50) of mercury (0.026ppm, 0.040ppm and 0.080ppm) and chromium (3.4ppm, 5.1ppm and 10.2ppm) for 28 days in Clarias batrachus and the protein content in the brain, gills, liver, kidney and muscle were quantified Bradford method. The levels of protein content in the liver, kidney, brain, muscle and gill showed fluctuations of decreasing and increasing trend in fishes exposed to different concentrations of mercury and chromium in a time–dose dependent manner.The protein variation in the tissues of C.batrachus were significant when exposed in mercury and chromium. The variations observed in the total protein quantified in the C.batrachus as a general indicator of pollutant induced stress response is discussed.