Methodological adjustments to the vigor tests in Brachiariabrizantha Marandu cultivar seeds

The objective of this study was to adjust the accelerated aging and electrical conductivity tests, to evaluate the physiological potential of lots from Brachiariabrizantha Marandu cultivar seeds, represented by four lots. For the accelerated aging test, seeds were submitted to traditional accelerated aging and with saturated NaCl solution and five periods of aging, 0, 24, 48, 72 and 96 h. For the electrical conductivity test, seeds were submitted to 12 periods of imbibitions in water for 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 h, using samples of 25 seeds in 25 mL and 50 mL, 50 seeds in 50 mL and 75 mL of water. To characterize the profile of lots, were determined moisture content, germination tests, first count of germination, speed of germination, initial stand, emergence, and speed of emergence index. The accelerated aging test makes possible the separation of lots of B. brinzathacv Marandu by the method with saturated NaCl solution for 72 h. For the electrical conductivity test, the imbibition period during 14 h using 50 seeds in 50 mL to cv Marandu is appropriate for evaluating the seed physiological potential.