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Optimising nutrients enhancing biomass and lipid content of Nitzschia palea in CSI Media

Author: 
Josephine Jenifa Lourduraj and David Ravindran Abraham
Subject Area: 
Life Sciences
Abstract: 

Effect of different carbon source, nitrogen source and different concentration of silica, iron and salt in the growth medium on growth, biomass and lipid content of N. palea (Diatom) were investigated. The addition of high concentration of iron in Csi media significantly increase the growth, biomass and lipid content of N. palea. 0.05% (glucose), 0.05% (urea and nitrogen starvation), silica (100 mg/L and 20 mg/L), Ferric ethylene diamine tetra acetate (0.026Mm and 0.023mM) and salt (20ppt and 40ppt) showed a marginal rise in biomass and lipid content respectively. Among these, silica concentration and salt concentration played a major role in yielding highest biomass (0.838 ± 0.019 g L–1 dw, 0.88 ± 0.018 g L–1 dw) as well as highest lipid content (68.5 ± 0.84 %, 66.7 ± 1.12%) respectively. The present work highlights the importance of optimising culture media (Csi) in enhancing both biomass and lipid content to provide N. palea as efficient stain for biodiesel production.

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