
Aim: To Investigate the Ayurvedic formulation for any sign of toxicity and biological evaluation. Materials and methods: The whole study was designed to carry out the research work on amnesia model in mice taking into consideration various parameters that were helpful analysed the potency of the test drug. The toxicity studies were also be performed on rodents prior to conduct the experimentation process in Morris Water Maze model. The study also includes the standardization of selected herbal formulation with various parameters like Ash value, extractive value, moisture content, pH and phytochemical investigation. Acute toxicity studies (50mg/kg, 300mg/kg and 2000 mg/kg orally of test sample)was conducted to determine the safe dose as per OECD-423 guidelines. Three Groups of animals (n=5) were used in this study. Group I was disease control group, Group II received Standard drug (Piracetam) and Group III received Test drug (Divya Medha Vati). Result: No sign of toxicity was observed. The test sample increases the latency time to find the hidden platform in M.W.M. Data of this method was analysed by annova and dunnetts multiple comparison at p<0.01. Conclusion: Results obtained in the present study suggest that the herbal formulation shows significant anti-amnesic activity when divya medha vati and piracetam were used separately.