Comparative evaluation of dentinal surface changes using tetracycline hydrochloride and ethylenediaminetetraacetic acid in fluorosed and non-fluorosed teeth with scanning electron microscope- An invitro study

Background: Fluorosis may change the mineralisation of the teeth. For this the first study was done by Vandana K L in 2007 to assess the dentinal surface changes in fluorosed teeth after chemical root biomodification. Aim: To compare dentinal surface changes in fluorosed and non-fluorosed teeth after application of EDTA and Tetracycline Hydrochloride. Materials and Methods: Thirty two human teeth extracted due to advanced periodontal disease and orthodontic treatment planning was used in this study. Also thirty two fluorosed teeth extracted due to periodontal disease and orthodontic treatment planning was used. The teeth were scaled and root planed. Sixty four dentin discs were prepared and divided into four groups like fluorosed healthy, non-fluorosed healthy, fluorosed diseased and non-fluorosed diseased. After, they were treated with EDTA and Tetracycline Hydrochloride. All the specimens were fixed and observed under scanning electron microscope and statistically analysed. Results: Overall smear layer score was more in FH group followed by NFH, FD, and NFD group in descending order with the specimen treated with TTC HCL. For degree of demineralization it was higher in FH followed by NFH and NFD were same and FD with specimen treated with TTC HCL. Conclusion: Fluorosed teeth show more degree of demineralization than non-fluorosed teeth.