Enhanced production of thermostable amylase by thermophilic geobacillus thermoleorans strain rekadwadsis isolated from unkeshwar hot spring sediment

The thermohilic Geobacillus thermoleorans Strain REKADWADSIS isolated from Unkeshwar thermal spring showed optimum temperature 65 ± 0.2 oC at its pH optima 7.5 ± 0.2. Geobacillus amylase production was detected on starch nutrient agar plates at 65 oC. Geobacillus was produced 8, 623 U/mL amylase under SmF at 68 ± 0.2 oC. The supplementation of additional lactose (1%) and tryptone (1%) with 10% inoculum was enhanced the production of thermostable amylase (14, 223 U/mL). Geobacillus thermostable amylase has apparent approximately MW 42 kDa.