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Enhanced production of thermostable amylase by thermophilic geobacillus thermoleorans strain rekadwadsis isolated from unkeshwar hot spring sediment

Author:

Bhagwan N. Rekadwad

Subject Area:

Life Sciences

Abstract:

The thermohilic Geobacillus thermoleorans Strain REKADWADSIS isolated from Unkeshwar thermal spring showed optimum temperature 65 ± 0.2 oC at its pH optima 7.5 ± 0.2. Geobacillus amylase production was detected on starch nutrient agar plates at 65 oC. Geobacillus was produced 8, 623 U/mL amylase under SmF at 68 ± 0.2 oC. The supplementation of additional lactose (1%) and tryptone (1%) with 10% inoculum was enhanced the production of thermostable amylase (14, 223 U/mL). Geobacillus thermostable amylase has apparent approximately MW 42 kDa.

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