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Isolation of genomic dna from portieria hornemannii

Author: 
Louis Cojandaraj, A. and Dr. Viji, M.
Subject Area: 
Life Sciences
Abstract: 

The Protocols followed for the isolation of genomic DNA from Portieriahornemannii have certain limitations due to the presence of polysaccharides and polyphenols in the sample. DNA isolated by these protocols is contaminated with a sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of Portieriahornemannii, which was free from contamination. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagents.

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