Antibacterial and antiadhesive properties of a biosurfactant isolated from lactobacillus rhamnosus against some bacteria causing uti in iraqi women

In this study, production of biosurfactant by Lactobacillus rhamnosus isolated from vagina of Iraqi healthy women was studied. The optimal condition of biosurfactant production including aeration, growing at different temperature for different incubation periods of times were studied. Anaerobic condition for 24 hours at 30°Cwas fitting to biosurfactant production. Additionally, biosurfactant was extracted by chloroform: methanol (2:1) and partially purified by acid precipitation, surface activity of crude and partial purified biosurfactant were studied, antibacterial, antiadhesion and antibiofilm activities were evaluated against some Urinary tract infectioncausative bacteria, including Escherichia coli, Klebsiella pneumonia, Burkholderia cepacia and Staphylococcus aureus isolated from urine samples of Iraqi women suffering from UTI. Crude biosurfactant showed surface activity higher than partial purified biodurfactant.Both crude and partial purified biosurfactant showed inhibitory effect against UTI causative bacteria at concentration (32) mg\ml against S.aureus, K.pneumonia and B.cepacia, and (64) mg\ml against E.coli, and showed inhibitory effect on adherence and biofilm formation of these bacteria, K.pneumoniawas more sensitive to biosurfactant follow by S.aureus, while B.cepacia was more resist.