Assessment of antibacterial activities of cotoneaster fruits extract

Methanol extract from cotoneaster fruit by using soxhlet apparatus was obtained. While aqueous extract was prepared by using magnetic heater. 1 g of solvent residue was dissolved in 10 ml of dimethyl sulphoxide, in same time, 1g of aqueous extract was dissolved in 10 ml of dimethyl sulphoxide, to obtain concentration of 100mg / ml, this depend for preparation of the 100, 50, 25 mg /ml for antimi-crobial activity assay for decoctions, for aqueous extract 1 g of air-dried plant material was added to 10 ml. of distilled water, left for 24 hours, mean while for alcoholic extract 1 g of powdered fruit added to 10 ml methanol (70 %) left for 24 hours. This 100 mg /ml depend for preparation of the 100, 50, 25 mg /ml for antibacterial activity assay. Isolated pure bacteria used in this study were: E. coli, Staphylococcus aureus, Bacillus subtlis, Pseudomonasspp and Proteus spp. The results showed that agar well diffusion method was more valuable to determine the antibacterial effects of both aqueous and alcoholic extracts of cotoneaster sp. In comparison with agar disc method, the alcoholic extract was more effective and showed higher antibacterial effect against all bacteria sp. In comparison with aqueous extract also both aqueous and alcoholic extract were concentration dependent as they were more effective at concentration 50 mg /ml in comparison with 25 mg /ml . at 100 mg /ml were highly effective in comparison with 25 and 50 mg ml the plants used in this study could be potential source of new antimicrobial agents.