Earthworms are one of the major soil biota but the application of insecticides in the agricultural field has been threatened the soil beneficial organisms including earthworms. Hence, in the present study sublethal concentrations of carbaryl (T1-4.195ppm and T2-13.984ppm) on the biochemical response of the L.mauritii was investigated. The activities of following enzymes were measured: superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) and acetyl cholinesterase (AChE). The levels of lipid peroxides (LP) and glutathione level (GSH) were also determined. The results showed that the GSH, SOD, CAT, GST and AChE activities were decreased than the control up to 15 days thereafter slightly increased on the 30 days. The results suggested that carbaryl was affected the earthworm populations in the soil.